Method for electroporation for the early chick embryo.
نویسندگان
چکیده
In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures, and some experimental examples are presented.
منابع مشابه
A detailed description of an economical setup for electroporation of chick embryos in ovo
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ورودعنوان ژورنال:
- Development, growth & differentiation
دوره 50 6 شماره
صفحات -
تاریخ انتشار 2008